Most certainly! It will work on any PC network that supports the TCP/Ip protocol.
All you need to do is to run the TLServer at the PC that connects to the PLCs via RS232/RS485, then any other PCs on your intranet will be able to access the PLCs, do programming or remote control or collect data into their Excel spreadsheet. The only things that other PC needs to know is the intranet IP address of the TLServer.