You are using DOS TRiLOGI within Windows XP which will not work. None of the 32-bit Windows allows DOS program to take control of the serial port directly (which the DOS program must do).
If you must run the DOS TRiLOGI then you need a way to boot into pure DOS mode. It may be possible if you have a floppy drive
and copy the system file to the diskette, then boot the PC into DOS mode and run the DOS TRiLOGI from there.
If your E10-Relay PLC is not "+" version then it would not work with WinTRiLOGI that can run under newer versions of Windows from XP onwards. So you will need to find an older computer that runs pure DOS or Windows 98 which allow booting into pure DOS mode.
If you still have your DOS TriLOGI source file you could purchase a new E10-Relay+ PLC and run the same program. All the E10 with "+" can work with WinTRiLOGI program.